Comparison between High Performance Liquid Chromatography and Capillary Electrophoresis in detecting Haemoglobin Constant Spring

Authors

  • Emida Mohamed Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA(UiTM), UiTM Kampus Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia; bDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia
  • Muhammad Ikmal Mohd Nizara aCentre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA(UiTM), UiTM Kampus Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia; bDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia
  • Raja Zahratul Azma Raja Sabudin Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA(UiTM), UiTM Kampus Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia; bDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia
  • Siti Nazrina Camalxaman Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA(UiTM), UiTM Kampus Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia; bDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia
  • Azlin Sham Rambely Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA(UiTM), UiTM Kampus Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia; bDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia
  • Norhisham Haron Centre of Medical Laboratory Technology, Faculty of Health Sciences, Universiti Teknologi MARA(UiTM), UiTM Kampus Puncak Alam, 42300 Bandar Puncak Alam, Selangor, Malaysia; bDepartment of Pathology, Faculty of Medicine, Universiti Kebangsaan Malaysia, 56000 Cheras, Kuala Lumpur, Malaysia

Keywords:

Capillary Electrophoresis, Haemoglobin Constant Spring (HbCS), High Performance Liquid Chromatography

Abstract

Two popular methods - High Performance Liquid Chromatography (HPLC) and Capillary Electrophoresis (CE) have been compared for diagnosis of Haemoglobin Constant Spring (HbCS). The performance of the methods was evaluated in terms of sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV). A total of thirty-two results were collected at HUKM which consisted of results derived from HPLC, CE and ARMS-PCR from December 2012 until December 2017. Results obtained showed CE demonstrated higher sensitivity, specificity, PPV and NPV compared to HPLC in the detection of HbCS.

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Published

2020-07-29

How to Cite

Mohamed, . E. ., Mohd Nizara, M. I. ., Raja Sabudin, R. Z. A. ., Camalxaman, . S. N. ., Rambely, . A. S. ., & Haron, . N. . (2020). Comparison between High Performance Liquid Chromatography and Capillary Electrophoresis in detecting Haemoglobin Constant Spring . Healthscope: The Official Research Book of Faculty of Health Sciences, UiTM, 1. Retrieved from https://healthscopefsk.com/index.php/research/article/view/26

Issue

Vol. 1 (2019): Healthscope

Section

Articles