Immuno-Positive Stained of Ki-67 from Formalin-Fixed Paraffin-Embedded Serous Cystadenoma and Serous Epithelial Ovarian Carcinoma Specimens

Authors

  • Rima Melati Mat Satar
  • Nor Salmah Bakar
  • Hartini Yusof
  • Maimunah Mustakim UNIVERSITI TEKNOLOGI MARA

Keywords:

Formalin-fixed paraffin-embedded samples, Immunohistochemistry, Ki-67 expression, ovarian carcinoma

Abstract

A vast archive of biopsy and autopsy specimens processed for histological diagnosis exists in pathology laboratory worldwide has potential value for immunohistochemistry studies associated with protein identification such as Ki-67. Determination of cellular activity has been reported to be of a diagnostic with prognostic value used to estimate the number of proliferating cells in ovarian carcinoma. Ki-67 antigen was found to be a reliable diagnostic marker for estimating the Proliferative Index (PI) of a neoplastic lesion by the Mindbomb E3 ubiquitin protein ligase1 (MIB-1 antibody) using immunohistochemistry. However, there is lack of published studies about Ki-67 from serous epithelial ovarian carcinoma (SEOC) in Malaysia. Thus, the purpose of this study is to identify immuno-positive stained associated with protein expression of Ki-67 from serous cystadenoma and SEOC specimens. FFPE specimens were sectioned and subjected to immuno-histo staining using monoclonal M1B1 antibody. Immuno-positive stained for Ki-67 was analysed by the blinded consultant pathologist. The result was presented as percentage of Ki-67 index among the samples studied. Ki-67 positive stained-samples were highest in SEOC. This study showed that archive FFPE samples were useful to be used for immunohistochemistry study.

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Published

2021-10-30

How to Cite

Mat Satar, R. M. ., Bakar, N. S. ., Yusof, H., & Mustakim, M. (2021). Immuno-Positive Stained of Ki-67 from Formalin-Fixed Paraffin-Embedded Serous Cystadenoma and Serous Epithelial Ovarian Carcinoma Specimens. Healthscope: The Official Research Book of Faculty of Health Sciences, UiTM, 4(1), 150-152. Retrieved from http://healthscopefsk.com/index.php/research/article/view/250

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