Immuno-Positive Stained of Ki-67 from Formalin-Fixed Paraffin-Embedded Serous Cystadenoma and Serous Epithelial Ovarian Carcinoma Specimens
Keywords:Formalin-fixed paraffin-embedded samples, Immunohistochemistry, Ki-67 expression, ovarian carcinoma
A vast archive of biopsy and autopsy specimens processed for histological diagnosis exists in pathology laboratory worldwide has potential value for immunohistochemistry studies associated with protein identification such as Ki-67. Determination of cellular activity has been reported to be of a diagnostic with prognostic value used to estimate the number of proliferating cells in ovarian carcinoma. Ki-67 antigen was found to be a reliable diagnostic marker for estimating the Proliferative Index (PI) of a neoplastic lesion by the Mindbomb E3 ubiquitin protein ligase1 (MIB-1 antibody) using immunohistochemistry. However, there is lack of published studies about Ki-67 from serous epithelial ovarian carcinoma (SEOC) in Malaysia. Thus, the purpose of this study is to identify immuno-positive stained associated with protein expression of Ki-67 from serous cystadenoma and SEOC specimens. FFPE specimens were sectioned and subjected to immuno-histo staining using monoclonal M1B1 antibody. Immuno-positive stained for Ki-67 was analysed by the blinded consultant pathologist. The result was presented as percentage of Ki-67 index among the samples studied. Ki-67 positive stained-samples were highest in SEOC. This study showed that archive FFPE samples were useful to be used for immunohistochemistry study.